Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -------------------------MVMLSLSVFFKGLGIGSGLI------VAIGAQNAFVL------KQGLKQQYVFWLCLICALSDSILIACGVLGFAEIMTASPILITVAKYLGAT---FLFVY-----GAKAFYAAF-----KTNQGMELDSSQ-KQTLTQA---LITCLAFTWLNPHVYLDTIVLIGSVATQLEDKISFALGSILASWIFFFSLGYGARFLKPLFINPKAWKILDFMIGCVMWSIAISL-LI----------- 4ECF Chain:A ((2-261)) AGESITAVGSTALQPLVEAAGEQYTGEHLGTFINVQGGGTGTGLSQIQEGAVQIGNSDLFAGEQKGINARQLVDHRVAVVGITPIVNKKVGV--KNLSTNQLIKIFTGQITNWKEVGGADQSIVLINRAQGSGTRATFEQFGLANHRSKTAQEQDSSGMVRSIVATTPGAISYVAFSYVNKTVQALSLNHVAPTEVNVTTN-DWRIWSYEHLYTKGHPTGLTKAFITYVQSPAIQNTLVRQL-----GYLSPDQMLVERDANGHITKT

General information: TITO was launched using: RESULT: Template: 4ECF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1005 -76604 -76.22 -394.87 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -76.22 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.250

(partial model without unconserved sides chains): PDB file : Tito_4ECF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ECF-query.scw PDB file : Tito_Scwrl_4ECF.pdb: