Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNQKFKLPLAIAIASAVLVGCSSNKVKEAKPNPLPKLTESNKSLVPVFSRSVSSTNKADPLRLQLDASEGVVFTLDPKGEVAAY--RGKQRLWEKKVSKL---------GLSSGVEAAEGIVVVGNSKGQLFALDQATGEQKWTAQLSGA-----LLSPSLVQSGRVITIANDGTVFAHDVSSGQQVWAYKLPNVQF-SLRGQPSPVS-LDPRTVLIASA-NAYVYAIDTISGIPHFQRRVAVSEGRSDIQR------------------LIDIDGDPVVAGQLMVTT--SFQGQVTVTDLASQRVVWSEDSSSTK---------------------------------------RPEVY--DNKVFVSS---------------------------------TDGKLTAYDLTTGEQLWQNDSLLNRHLSNPVVLGSDLIVGDLDGVLHLIDPTTGKLIGRSKTSGEV--NTLRV-IEKQLYVSTRKGDLSIWQNR 4MH1 Chain:B ((46-528)) -----------------------------------------GQLQLVWARGMQPGK----VQVTPLIHDGVMYLANPGDVIQAIDAKTGDLIWEHRRQLPNIATLNSFGEPTRGMALYGTNVYFVSWDNHLVALDMGTGQVVFDVDRGQ-DERVSNSSGPIVANGTIVAGSTGCFVSGHDSATGEELWRNYFI----WMTGAWGQITYDPVTNLVHYGS-GTNTRFAVRPDTGEIVWRHQTLPRDNWDQECTFEMMVTNVDVQPSTEMEGLQSINPNAATGERRVLTGVPCKTGTMWQFDAETGEFLWARDTNYQNMIESIDENGIVTVNEDAILKELDVEYDVCPTFLGGRDWPSAALNPDSGIYFIPLNNVCYDMMAVDQEFTSMDVYNTSNVTKLPPGKDMIGRIDAIDISTGRTLWSVERAAANYSPVLSTGGGVLFNGGTDRYFRALSQETGETLWQTRLATVASGQAISYEVDGMQYVAIAGGG-------

General information: TITO was launched using: RESULT: Template: 4MH1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1859 13148 7.07 40.71 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : 7.07 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.446

(partial model without unconserved sides chains): PDB file : Tito_4MH1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4MH1-query.scw PDB file : Tito_Scwrl_4MH1.pdb: