Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNLQNKLKHLEKDNHMSLSDEEQFDSLKSFAKKYGSAMISGILIALIAFFGWEYWQKRNLATSQTETAKVQQLMDEANATADNPNALASITASADKIVKDDIDSVQAIQTQFVLAKLAYEKQDYAAAEKALKKVENSKVKDEG-LIQV--VKLRLADAQLAQNK----YDEALKTLSGDVDPAFKATVEELRGDIFVAKKDIDSAKKAYQAAWDSLLERKQERQILQIKLESVGVLVEDPQIERPILETQVEES 4D18 Chain:B ((153-282)) ------------------------------------------------------------------------------------------------------------FKTNTKLGKLYLEREEYGKLQKILRQLHQSCQT---QLLEIYALEIQMYTAQKNNKKLKALYEQSLHIKSAIPHPLIMGVIRECGGKMHLREGEFEKAHTDFFEAFKNYDESGSPRRTTCLK-------------------------

General information: TITO was launched using: RESULT: Template: 4D18.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 379 -858 -2.26 -7.66 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -2.26 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.562

(partial model without unconserved sides chains): PDB file : Tito_4D18.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D18-query.scw PDB file : Tito_Scwrl_4D18.pdb: