Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACQQPIRWYQNIPVISWLTLRGKCGHCQHPISIRYPAVELLTMLCSLVVVMVFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDRFTLTLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSLLGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG 5FLM Chain:L ((16-44)) ----------------------------------------------------------------------IYICGECHTENEIKS-----R---DPIRCRECGYRIM-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5FLM.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 81 -10463 -129.17 -360.79 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain L : 0.36 3D Compatibility (PKB) : -129.17 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.36 QMean score : 0.609

(partial model without unconserved sides chains): PDB file : Tito_5FLM.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FLM-query.scw PDB file : Tito_Scwrl_5FLM.pdb: