Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKLNALLCSAVLATSMVTVAHADNTTHVAAASALGSIAGTAIGKSMGGTNGGYIGAALGAAGGSALGNKISKDRDAD--------RSSKYWKKNTVIIVTNYRDEIGLKTPSGVFL 5EZ4 Chain:A ((225-286)) ----------------------------------------------------GTINLILGA--GSEVGDVMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIF-

General information: TITO was launched using: RESULT: Template: 5EZ4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 158 7981 50.51 147.79 target 2D structure prediction score : 0.35 Monomeric hydrophicity matching model chain A : 0.57 3D Compatibility (PKB) : 50.51 2D Compatibility (Sec. Struct. Predict.) : 0.35 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.126

(partial model without unconserved sides chains): PDB file : Tito_5EZ4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5EZ4-query.scw PDB file : Tito_Scwrl_5EZ4.pdb: