Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQSGGFLVMCPFCGRS-------------KGGLVCGYCGWVSKDKKFRFVVVKNG 1VD4 Chain:A ((11-48)) ---NRASFKCPVCSSTFTDLEANQLFDPMTGTFRCTFCHTE--------------

General information: TITO was launched using: RESULT: Template: 1VD4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 36 -4460 -123.88 -178.38 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -123.88 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.768

(partial model without unconserved sides chains): PDB file : Tito_1VD4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VD4-query.scw PDB file : Tito_Scwrl_1VD4.pdb: