Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTDITLKTPVEMRNVQLKVYDNTDNSILIDTQVASLT-S---YDIKLPSTKLNRLYRIEVTTQ--GSSQVFDPTKSEYQNISGVYHAFITPSSISNKTQLISPSSEAIYQRAVIRSGQLPNETIIPTRIEQLHVD--LASQDVYRSLLNAFKDTNIPSLSPASTLTILNLLKYSTTKPSTYVDSYLCFGYLQYWSSIHNTNSPYQDLTQSLATDLKDGYLDGKKLIGDKTSFTPIFTSAPDNIDPAKNTLLDIAANQKVTR-DYFATNLRAATFKLADLQDQSHLDPIGY-DLLDKKQYSGIIPAGNTSEFFRIDGAGDYRRAVGFSGITATCNGSAYPCKQGLTSINISDPKLPATDYLVGHYEDSTTGCQLNFRANGQIELIKGSQSYRSALSADSTDKLLQTDAASYSYLLNSSSSEPNNSTQQYNFIQLHIKANKIVSAQAGLDSRKAPDVLQT-TQLEYSFS 5T1P Chain:A ((22-347)) ------------------IDENLIKAAQAEGRVNSLAMPDTWANWKDTWADLKNLYDIEHSDTDMSSAQEIAKFKTEKKNASG------------DIGDVGASFGEIAVKQGVAQPFKTSYWDQIPTWAKDKDGNWLLAYTGTIAFIVNKDVVKDIPK-------TWQDLLKGN------------------YKITVGDVSVAAQAVSAVLAANYALG--------GDEK-----------DLSPALAFFNTLAKQGRLVNNDVSIANLEKGEVEVGLVW---DFNGLGYRDKVGKDRYEVLIPA---------DG----------------------SVISGYTTIINKYAKHPNAAKLAREFILS---------DKGQINLAKG---YARPIRIDHIT-------------LPDDIKAKLLPSEQYKNARAIKDQKAWEKSAKELPQLWQEKVIVDMK-------

General information: TITO was launched using: RESULT: Template: 5T1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1851 114388 61.80 363.14 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : 61.80 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.160

(partial model without unconserved sides chains): PDB file : Tito_5T1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5T1P-query.scw PDB file : Tito_Scwrl_5T1P.pdb: