Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKVEQNEGLVEKLVAVDRVAKVVKGGRIFSFTALTVVGDGNGRVGFGRGKAREVPAAISKALEAARRNMITVDLAGTTLQHPVNARHGASRVYMQPASEGTGVIAGGAMRAVLEAAGVHNVLAKCYGSTNAANVVNATFKGLRDMTSPEKVAAKRGKSVEEIQG 5O5J Chain:E ((37-189)) ----------IERVVSINRVSKVVKGGRRFSFTALVIVGDGKGMVGVGYGKAKEVPAAIAKGVEEARKNFFRVPLIGSTITHPVQGEAAAGVVMLRPASPGTGVIAGGAARAVLECAGVHDILAKSLGSDNAINVVHATVAALKLLQRPEEVAARRGLPIEDV--

General information: TITO was launched using: RESULT: Template: 5O5J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 782 -95799 -122.51 -626.14 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain E : 0.89 3D Compatibility (PKB) : -122.51 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.89 QMean score : 0.560

(partial model without unconserved sides chains): PDB file : Tito_5O5J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5O5J-query.scw PDB file : Tito_Scwrl_5O5J.pdb: