Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----------------------MRLMINKSMKNIYIMGLLLACSG-FAMAETVTQQPTGQPVRTASNPNAIRIVTR---------PEIMGLWGMEIPNN--------KKCVEYYNFRSSNDVVIKSGEEWS---YGIY-------EY--QPSDDPKEQLSALVMQIKFDNNKVDCSGQKQDQTGDISQYFVQWKNDHTINFCSTAKGEQCFATLRRMLP 5L2D Chain:A ((19-252)) PMWVDFSDTASMKNLDPQGGFKVGTVFKKEISPGYVVTLTVTELKPFNSTEIYKKRVEGTPTANTYDPNAINSYLKGYKDYGKTPPSVTGRTQIILPDDAVNWGIKFKVEATYRGNPVKPSVVMADGEDANPAEYGIFTTNGEGWEYVGEWMKGPYTVMT-EDMVKAFDKTRKDGLLILKDKSVDWSKYL-------SPDTVTGGLGSQVFGPIISAS-

General information: TITO was launched using: RESULT: Template: 5L2D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 547 32581 59.56 206.21 target 2D structure prediction score : 0.51 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : 59.56 2D Compatibility (Sec. Struct. Predict.) : 0.51 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.151

(partial model without unconserved sides chains): PDB file : Tito_5L2D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L2D-query.scw PDB file : Tito_Scwrl_5L2D.pdb: