Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFMTESRLTHLKQLEAESIHIIREVAAEFENPVMLYSIGKDSAVMLHLALKAFYPAKLPFPLLHVDTGWKFKDMIAFRDNMAKTHGF----DLIVHQNKEG--RE-----AGINPFDHGSSKYTDIMKTQALKQALDKYQFDAAFGGARRDEEKSRAKERVYSFRDSKHRWDPKNQRPELWNLYNGKVNKGESIRVFPLSNWTELDIWQYIYLENIQIVPLYFSAVRPVVERSGTLIMVDDERMRLKEGEVPQMKSVRFRTLGCYPLTGAVESEADTLPEIIQEMLLATSSERQGRMIDHD-EAGSMEKKKQEGYF 2OQ2 Chain:C ((29-256)) ----------------TPQEIIAWSIVTFPHLFQTTAFGLTGLVTIDMLSKLSEK-YYMPELLFIDTLHHFPQTLTLKNEIEKKYYQPKNQTIHVYKPDGCESEADFASKYGDFLWEKDDDKYDYLAKVEPAHRAYKELHISAVFTGRRKSQGSARSQLSIIEID-----------------------ELNGILKINPLINWTFEQVKQYIDANNVPYNELLDL---------------------------------GYRSIGDYHSTQPVKEG---------------EDERAGRWKGKAKTECGIHEASRFAQF

General information: TITO was launched using: RESULT: Template: 2OQ2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1053 7596 7.21 35.16 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain C : 0.66 3D Compatibility (PKB) : 7.21 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.382

(partial model without unconserved sides chains): PDB file : Tito_2OQ2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2OQ2-query.scw PDB file : Tito_Scwrl_2OQ2.pdb: