Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------------------------------------------------------------MSHNLITTKTEFSQMPID--TQLTTLFCLIDDFCTDITQNVEQYMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAG--IAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFK---------IHLICDHIGRLVSYCITTGNV-------DDRKVLPDLI-------EHSKLKGKLFGDRGYVGKNWKSRLAEVG-----VQLITRVKRNMKPQVLAPFDHAVLKKRGIIEAPFKLMKSQFDLEHSRHRSKMGLL------TTIFAALTLYALVLVNGYKSGIQQILKPIDLNSA-------------------- 2EUA Chain:A ((2-429)) QSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNGDEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRC------GGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGN--VVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQ

General information: TITO was launched using: RESULT: Template: 2EUA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1112 -24588 -22.11 -80.09 target 2D structure prediction score : 0.34 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : -22.11 2D Compatibility (Sec. Struct. Predict.) : 0.34 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.124

(partial model without unconserved sides chains): PDB file : Tito_2EUA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EUA-query.scw PDB file : Tito_Scwrl_2EUA.pdb: