Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFASILRELNYLRSNKWDSCMVTLIPAFIIFLFSSMFYAGKAEHLPIAIIDQDQSELSRNIGKYLSLNHTVDVKTITTSSSEAERLLNETKIWGYIFIPDGAEKRFVKAQDAQISIAFNQSFFSVGNTISSAMLVSTLNALADYAGQNYLANNIPYLDVPTAHVKISTLYNPSMSYEFYLEPFMVPAVLHLLLCCCVAFAIGQEIKRGTLTQW-VNRE-SFIQGLLAKNLLYSFIFCFWTWLWMFWLVEIRGWFV-AGSLSFLLAAQFLLYFSYALISSTVILATKNLSKTFGFIAVYGGSSLSFAGVTLPLNNAPIFTKFWSLIIPYTPYAKLQTEQWVIGSPL------------------------FISMSPFLILIGYCLIYFFLSCLLLKKLVQGATP 5DO7 Chain:B ((472-671)) ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------KCYSERAMLYYELEDGLYTTGPYFFAKILGELPEHCAYIIIYGMPTYWLANLRPGLQPFLLHFLLVWLVVFCCRIMALAAAALLPTFHMASFFSNALYNSFYLAGGFMINLSSLWTVPAWISKVSFLRWCFEGLMKIQFSRRTYKMPLGNLTIAVSGDKILSVMELDSYPLYAIYLIVIGLSGGFMVLYYVSLRFIKQKP--

General information: TITO was launched using: RESULT: Template: 5DO7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 525 -96519 -183.85 -557.91 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain B : 0.53 3D Compatibility (PKB) : -183.85 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.269

(partial model without unconserved sides chains): PDB file : Tito_5DO7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DO7-query.scw PDB file : Tito_Scwrl_5DO7.pdb: