Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSNLTLSQFLQQE--KGNLTPELAQVIDTIAATCKTIDQALQKGALAGILGSAGNENVQGETQKKLDVISNDYLIDALKVHPHVGGLASEELDD--FTPAQENGEYLVLFDPLDGSSNIDINMCVGTIFSILPAKNAVTQA--QDFMQAGTQQVAAGYVLYGPSTMMALTVGNGVAFFTLDPETQTFLLTTENVQVSADTQEFAINASNQRHWEQPVKQYIEELLAGKTSVREK-------DFNMRWVACMVGDVHRILCRGGIFLYPYDLKDPKKAGRLRLMYEANPMSMLIEQAGGASTTGRVRILEIEPTELHQRVPVIIGSKNEVERVTSYH 4GWW Chain:A ((11-324)) ---VTLTRFVKEEGRKARGTGEMTQLLNSLCTAVKAISTAVRKAGIAH--------------VKKLDVLSNDLVINVLKSSFATCVLVSEEDKNAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSIGTIFGIY-RKNSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMVNGVNCFMLDPAIGEFILVDRDVKIKKKGSIYSINEGYAKEFDPAITEYIQ---------RKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFMYPANKKSPK--GKLRLLYECNPMAYVMEKAGGLATTGKEAVLDIVPTDIHQRAPIILGSPEDV-------

General information: TITO was launched using: RESULT: Template: 4GWW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1569 19224 12.25 66.98 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.83 3D Compatibility (PKB) : 12.25 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.481

(partial model without unconserved sides chains): PDB file : Tito_4GWW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4GWW-query.scw PDB file : Tito_Scwrl_4GWW.pdb: