Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence -----------------MKNKNIGCWLLLAGSSSVCAMQPLDDQSLAAATGQN---GLTLGIQADQ----------VKFN----QVALIDTNG--------IASTSYNSKAGLIIAGNSTNPVPGI-------EFIKAAVSTN-PSFNIAI---DTDAGGGNPFLNLAVTMGSDVNGIRLLPFSVYLAPSTSLPSPSDYALTSYAP-----KSIFSSGTTVNTGVKELI-------RSTGN---LDINFVQT-----NKPRLNIQLGHAAQSVMVKFG--GAIQSICSAASGCPITLVSDNTGATFGFKFAGTNAST----GFVLDG----------FYAGVD------------PTGLTIGNIGVSSKFDASLNNVTLGNLGTQSTTTFNNLPNGSMGSFGVTGASVTDFKMKVSGF---------------------------- 1F60 Chain:A ((2-441)) GKEKSHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYQVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDSVKWDESRFQEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEATTNAPWYKGWEKETKAGVVKGKTLLEAIDAIEQPSRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVIKPGMVVTFAPAGVTTEVKSVEMH-HEQLEQGVPGDNVGFNVKNVSVKEIRRGNVCGDAKNDPPKGCASFNATVIVLNHPGQISAGYSPVLDCHTAHIACRFDELLEKNDRRS-GKKLEDHPKFLKSGDAALVKFVPSK----PMCVEAFSEYPPLGRFAVRDMRQTVAVGVIKSVDK

General information: TITO was launched using: RESULT: Template: 1F60.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1506 9571 6.36 30.77 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 6.36 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.198

(partial model without unconserved sides chains): PDB file : Tito_1F60.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1F60-query.scw PDB file : Tito_Scwrl_1F60.pdb: