Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---MLRILKSILFEFEELMSQQSVEKKSVPWL-----LMGLGLL---------IPFLIIG----------ILFLAAKSDAQNKKQY------EQQRLEMEKRIAEKEAANSDKK 5WTQ Chain:A ((13-126)) GDVSLHNFSARLWEQLVHFHVMRLTDSLFLWVGATPHLRNLAVAMSSRYDSIPVSTSLLGDTSDTTSTGLAQRLARKTNKQVFVSYNLQNTDSNFALLVENRIKEEMEAFPEKF

General information: TITO was launched using: RESULT: Template: 5WTQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 278 1462 5.26 18.05 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 5.26 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.228

(partial model without unconserved sides chains): PDB file : Tito_5WTQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5WTQ-query.scw PDB file : Tito_Scwrl_5WTQ.pdb: