Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGRRKQGMPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKRGVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKDLPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA 5MY1 Chain:L ((2-123)) ---------TVNQLVRKPRARKVAKSNVPALEA-------------CPQKRGVCTRVYTTTPKKPNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGALDCSGVKDRKQARSKYGVKRPKA

General information: TITO was launched using: RESULT: Template: 5MY1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain L - contact count / total energy / energy per contact / energy per residue : 497 -18254 -36.73 -149.62 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain L : 0.90 3D Compatibility (PKB) : -36.73 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.90 QMean score : 0.491

(partial model without unconserved sides chains): PDB file : Tito_5MY1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MY1-query.scw PDB file : Tito_Scwrl_5MY1.pdb: