Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAGDEGVTPPVDPENPDPNK---PVDPSDPPSPG-TGGALSIDYGSKFKFGTQKISTADKTYYAAADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVSLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVPGKSVKLAQQYSTKLVWTLEDTPNN 1FBW Chain:A ((113-195)) ------------------------------------------------------PEFQDPSKYPSPLDTSQPVGRDPINSQLTSAYGTSMLYGMHWILDVDNWYGFGARA--DGTSKPSYINTFQRGEQESTWE-TIPQP-----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1FBW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 154 16028 104.07 202.88 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 104.07 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.329

(partial model without unconserved sides chains): PDB file : Tito_1FBW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1FBW-query.scw PDB file : Tito_Scwrl_1FBW.pdb: