Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ML----------PFFIYWGVILACIA------WLALSVYFSVFYLVRKEN----GNL-WAFALFNVI-----AAIVLAITLAVYR---TWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA---------- 2JOZ Chain:A ((1-135)) MIMVSGCQQQKEETPFYYGTWDEGRAPGPTDGVKSATVTFTEDEVVETEVMEGRGEVQLPFMAYKVISQSTDGSIEIQYLGPYYPLKSTLKRG--ENGTLIWEQNGQRKTMTRIESKTGREEKDEKSKSLEHHHHHH

General information: TITO was launched using: RESULT: Template: 2JOZ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 269 -10088 -37.50 -105.08 target 2D structure prediction score : 0.11 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -37.50 2D Compatibility (Sec. Struct. Predict.) : 0.11 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.183

(partial model without unconserved sides chains): PDB file : Tito_2JOZ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2JOZ-query.scw PDB file : Tito_Scwrl_2JOZ.pdb: