Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MPKFLSIIRI-RKKAKTFFVFAPTKMFQLFSFFIPVFLIFRMTG------------------------------------------------------------------ 2EE3 Chain:A ((1-108)) GSSGSSGLAPPRHLGFSDVSHDAARVFWEGAPRPV-----------RLVRVTYVSSEGGHSGQTEAPGNATSAMLGPLSSSTTYTVRVTCLYPGGGSSTLTGRVTTKKAPSPSSGPSSG

General information: TITO was launched using: RESULT: Template: 2EE3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 36 -2161 -60.01 -67.52 target 2D structure prediction score : 0.31 Monomeric hydrophicity matching model chain A : 0.53 3D Compatibility (PKB) : -60.01 2D Compatibility (Sec. Struct. Predict.) : 0.31 1D Compatibility (Hydrophobicity) : 0.53 QMean score : 0.220

(partial model without unconserved sides chains): PDB file : Tito_2EE3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EE3-query.scw PDB file : Tito_Scwrl_2EE3.pdb: