Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAVVGIPVGIMISRRRKLANWVIRLA-----NIIQTIPSLAMISILIIGL-GLGVNVVIVTVFLYSLLPIIKNTY------TGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIGITAIGAFVGAGGL---GDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPASRTSR 4YMV Chain:D ((15-214)) ---------------------------FISGLIMTLKLTFLAVTIGVLMGLFIALMKMSSIKPIKLVASSYIEVIRGTPLLVQLLLIYNGLMQFGMNIPAFTAGV-SALAINSSAYVAEIIRAGIQAVDPGQNEAARSLGMTHAMAMRYVIIPQAIKNILPALGNEFIVMLKESAIVSVIGFADLTRQADIIQSVTYRYFEPYIIIAAIY--FVMTLTFSKLLSLFERRL--------

General information: TITO was launched using: RESULT: Template: 4YMV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 698 -61528 -88.15 -332.58 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain D : 0.69 3D Compatibility (PKB) : -88.15 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.286

(partial model without unconserved sides chains): PDB file : Tito_4YMV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YMV-query.scw PDB file : Tito_Scwrl_4YMV.pdb: