Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKMKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIELTFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETAEMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGP-SIISAFKAPMPQVNIMPTGGVSLDNMKEWFDAGVVTVGVGGNLLAPA---ATGDFDKVTEVAQQYAAKMKEIKR 1EUA Chain:A ((27-206)) ---------------------------EHAVPMAKALVAGGVRVLEVTLRTECAVDAIR---AIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCPTGGISPANYRDYLALKSV-LCIGGSWLVPADALEAGDYDRITKLARE----------

General information: TITO was launched using: RESULT: Template: 1EUA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 847 -82669 -97.60 -469.71 target 2D structure prediction score : 0.78 Monomeric hydrophicity matching model chain A : 0.76 3D Compatibility (PKB) : -97.60 2D Compatibility (Sec. Struct. Predict.) : 0.78 1D Compatibility (Hydrophobicity) : 0.76 QMean score : 0.593

(partial model without unconserved sides chains): PDB file : Tito_1EUA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EUA-query.scw PDB file : Tito_Scwrl_1EUA.pdb: