Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSVIVLAGTIGAGKSSLT----------EMMAEHFDSQAFYESIDD---------NEVLPLFYANPEQYAFLLQIYFLNKR----FASIKQAMKD-DNNVL--DRSIYEDSLLFHLNA-DLGRATETEVRVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVAI 4JLN Chain:A ((44-235)) ---ISIEGNIAAGKSTFVNILKQLSEDWEVVPEPVARWSN------ELTMEQKNGGNVLQMMYEKPERWSFTFQTYACLSRIRAQLASLNGKLKDAEKPVLFFERSVYSDRYIFASNLYESESMNETEWTIYQDWHDWMNNQF---GQSLELDGIIYLQATPETCLHRIYLRGRNEEQ----GIPLEYLEKLHYKHESW-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 4JLN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 601 13276 22.09 85.10 target 2D structure prediction score : 0.62 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : 22.09 2D Compatibility (Sec. Struct. Predict.) : 0.62 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.499

(partial model without unconserved sides chains): PDB file : Tito_4JLN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4JLN-query.scw PDB file : Tito_Scwrl_4JLN.pdb: