Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDGLGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRFTLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDTIVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGLRGSKASVNKFKPDLFFAVDCSAADDT---VTKNGTFGHLGEGTLMRIQDPGLIMLPRLREYLLDIAETNNIPYQYFV-SKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFEAAREMLIQTLKGLDKSTVNTIVYGK 5L6Z Chain:A ((5-329)) --------IRKLTEAFGPSGREEEVRSIILEELEGHIDGHRIDGLGNLIVWKGSGEK---KVILDAHIAEIGVVVTNVDDKGFLTIEPVGGVSPYMLLGKRIRFENGTIGVVGMEGETTEER----QENVRKLSFDKLFIDIGANSREEAQK--MCPIGSFGVYDSGFVEVSGK-YVSKAMDDRIGCAVIVEVFKRIKPAV---TLYGVFSVQEEVG--------YGVPADEAIAIDVTDSADTPKAIKRHAM--RLSGGPALKVKDRASISSKRILENLIEIAEKFDIKYQMEVLTFG-------------IPSATVSIPTRYVHSPSEMIAPDDVEATVDLLIRYL---------------

General information: TITO was launched using: RESULT: Template: 5L6Z.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1663 -149764 -90.06 -499.21 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.77 3D Compatibility (PKB) : -90.06 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.77 QMean score : 0.442

(partial model without unconserved sides chains): PDB file : Tito_5L6Z.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5L6Z-query.scw PDB file : Tito_Scwrl_5L6Z.pdb: