Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGKVSDIEGLIEKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYSLRLAQEISYIYGFKDLMGENGELSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLSMKPMATKLQTELGKNVAYTEESLQQDLDILEGEFEEVSSDIASSDTVLSQLERLSHLLETNMITEEEFQQLKQELLKK 4YLF Chain:A ((108-161)) ----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------LVGGGVGIATLYPIAKALK-EAGNNITTVLGARTKDYLIMVDEFKEISDVLLVTD---------------------------------

General information: TITO was launched using: RESULT: Template: 4YLF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 171 -6036 -35.30 -111.77 target 2D structure prediction score : 0.22 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -35.30 2D Compatibility (Sec. Struct. Predict.) : 0.22 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.326

(partial model without unconserved sides chains): PDB file : Tito_4YLF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YLF-query.scw PDB file : Tito_Scwrl_4YLF.pdb: