Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTKIAVEKVSAEEARLMSTGFLFPGQGSQKLGMLADIPEMYMER---------LENVTGYRLVDKEENYQD-TVFIQLALLTKA-VFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVYHRAVLMKEAYPSGYA-MGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSIEGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKNTDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKFGVDKGIRTINLDQHGIDDATFLYQKWR 3EZO Chain:A ((0-281)) ----------------MKFAFVFPGQGSQSVGMLNAFADVAVVRETLDEASDALGQDIGKLIADGPADELNLTTNTQPVMLTAAYACYRAWQQAGGAQPSIVAGHSLGEYTALVAAGAIAFRDALPLVRFRAQAMQTAVPVGVGGMAAILGLDDDTVRAVCAEA-SATGVVEAVNFNAPAQVVIAGTKAGIEKACEIAKEKGAKRALPLPVSAPFHSSLLKPASDKLREYLAGVDVKAPKISVVNNIDVAVVSDPAAIKDALVRQAAGPVRWVECVQHIAREGVTHVIECGPGKVLAGL--------------------------------

General information: TITO was launched using: RESULT: Template: 3EZO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1539 -23520 -15.28 -87.11 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -15.28 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.432

(partial model without unconserved sides chains): PDB file : Tito_3EZO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3EZO-query.scw PDB file : Tito_Scwrl_3EZO.pdb: