Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEELAGEHRLFEKRNCDKTF-----VTVPSFFCYAILDVKNRRNKM-----REIYSFESKIYA----SEVGKGGAYIIFPYD-IRKEFGAGRVKVQATFDNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK 1GTA Chain:A ((131-213)) ---------KMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGG----------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1GTA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 153 1165 7.61 17.13 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.65 3D Compatibility (PKB) : 7.61 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.166

(partial model without unconserved sides chains): PDB file : Tito_1GTA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1GTA-query.scw PDB file : Tito_Scwrl_1GTA.pdb: