Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEFLKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYHIYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDL-GIAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIVFILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPIDWQLPETV 1SSP Chain:E ((1-221)) MEFFGE----SWKKHLSGEFGKPYFIKLMGFVAEERKHYTVYPPPHQVFTWTQMCDIKDVKVVILGQDPYHGPNQAHGLCFSVQRPVPPPPSLENIYKELSTDIEDFVHPGHGDLSGWAKQGVLLLNAVLTVRAHQANSHKERGWEQFTDAVVSWLNQNSNGLVFLLWGSYAQKKGSAIDRKRHHVLQTAHPSPLSVYRGFFGCRHFSKTNELLQKSGKKPIDWK-----

General information: TITO was launched using: RESULT: Template: 1SSP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain E - contact count / total energy / energy per contact / energy per residue : 1188 -32049 -26.98 -145.68 target 2D structure prediction score : 0.71 Monomeric hydrophicity matching model chain E : 0.84 3D Compatibility (PKB) : -26.98 2D Compatibility (Sec. Struct. Predict.) : 0.71 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.582

(partial model without unconserved sides chains): PDB file : Tito_1SSP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SSP-query.scw PDB file : Tito_Scwrl_1SSP.pdb: