Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNLEAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVPLAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALLFIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAAISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY 4LSG Chain:A ((704-739)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------WPYFVVGIFCAIIN-------GGLQPAFSVIF---SKVVGVFTNGG---------------

General information: TITO was launched using: RESULT: Template: 4LSG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 13 -1794 -138.00 -49.83 target 2D structure prediction score : 0.75 Monomeric hydrophicity matching model chain A : 0.43 3D Compatibility (PKB) : -138.00 2D Compatibility (Sec. Struct. Predict.) : 0.75 1D Compatibility (Hydrophobicity) : 0.43 QMean score : 0.171

(partial model without unconserved sides chains): PDB file : Tito_4LSG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4LSG-query.scw PDB file : Tito_Scwrl_4LSG.pdb: