Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKSTSTTSITNTSTGNVDSQHSQS--SIQSF--SISSTSEMQE-----STV---PSLPLENSDKALLFLIAHRSELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTPF 5DCA Chain:A ((1818-1912)) ----------------------------------SFIEIDDTEA-ETEIISTLSNGLIASHYGVSFFTIQSFVSSLSNTSTLKNMLYVLSTAVEFESVPLRKGDRALLVKLSKRLPLR-------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5DCA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 140 8475 60.53 119.36 target 2D structure prediction score : 0.38 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 60.53 2D Compatibility (Sec. Struct. Predict.) : 0.38 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.390

(partial model without unconserved sides chains): PDB file : Tito_5DCA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DCA-query.scw PDB file : Tito_Scwrl_5DCA.pdb: