Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVMLDPKNRQILTIGLAGKTNEDTRINVPIDDNFNTV--LKRIRSGENGIFERFRDNLLIEIVSYWNDQRLKNDEPTAQAVATPVTE-ETVSVPEEKTEKPKADTTENTRSTDTNAALSFADFSKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLTLIPLIEQFAATAITDR 4UTO Chain:A ((162-287)) --------------------------------------------DPNNKEFYEKNLKEYTDKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVPSAYIWEIN--TEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQIFTNSIAEQGK-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4UTO.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 374 40756 108.97 331.35 target 2D structure prediction score : 0.37 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : 108.97 2D Compatibility (Sec. Struct. Predict.) : 0.37 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.094

(partial model without unconserved sides chains): PDB file : Tito_4UTO.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4UTO-query.scw PDB file : Tito_Scwrl_4UTO.pdb: