Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQKRQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALGVDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGLKVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGP---------------REWYDDEFEVYGHYMPLDELLDQVDVMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADSLVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA 5G1P Chain:A ((56-311)) --------------------------------------------------SMFYEVSTRTSSSFAAAMARLGGAVLSFSEATSSVQKGESLADSVQTMSCYA-DVVVLRHPQPGAVE--LAAKHCRRPVINAGDGVGEHPTQALLDIFTIREELGTVNGMTITMVGDLKHGRTVHSLACLLTQYRVSLRYVAPPSLRMPPTVRAFVAS-GTKQEEFE------SIEEALPDTDVLYMTRIQKERF-------------QFILTPHIMTRAKKKMVVMHPMP--RVNEISVEVDSDPRAAYFRQAENGMYIRMALLATVL----

General information: TITO was launched using: RESULT: Template: 5G1P.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1101 -85919 -78.04 -375.19 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -78.04 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.458

(partial model without unconserved sides chains): PDB file : Tito_5G1P.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5G1P-query.scw PDB file : Tito_Scwrl_5G1P.pdb: