Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTAEKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQGGYTRILKTEPRRGDGAPMVVLELV 5MMI Chain:O ((19-126)) ----RLSRPADQRRALLRGLTTQLLKHGRIKTTRAKASAMRKYVDKMITLAKEGSLHKRRQALGFI----------YEKQIVHA----LFAEVPERYGDRNGGYTRIIRTLPRRGDNAPMAYIELV

General information: TITO was launched using: RESULT: Template: 5MMI.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain O - contact count / total energy / energy per contact / energy per residue : 372 -46074 -123.85 -426.61 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain O : 0.85 3D Compatibility (PKB) : -123.85 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.729

(partial model without unconserved sides chains): PDB file : Tito_5MMI.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MMI-query.scw PDB file : Tito_Scwrl_5MMI.pdb: