Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQIIDDVAGVTLASASTLDKEIS------GGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHGRVQALAEAARENGLEF 5X8T Chain:P ((53-166)) ----------RTARHVRIRKKVEGTPERPRLCVFRSNKHLYVQVIDDTKMHTLAAASTMQKAISENIDYSAGPTVEVAQKIGEMIAKSCLEKGITKVAFDRGGYPYHGRVKALADAAREHGLVF

General information: TITO was launched using: RESULT: Template: 5X8T.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 461 14983 32.50 138.73 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain P : 0.85 3D Compatibility (PKB) : 32.50 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.85 QMean score : 0.566

(partial model without unconserved sides chains): PDB file : Tito_5X8T.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5X8T-query.scw PDB file : Tito_Scwrl_5X8T.pdb: