Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWTIDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGLEFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYSSKVKYWMTFNEINSAFHFPALSQGLVKSNGARRVPKYFPSMA 4ZEN Chain:A ((30-184)) -------QVEGAWNEDGKGLSVWDV-------FAKQPGRTFKGT--------NGDVAVDHYHRYQEDVALMAEMGLKAYRFSVSWSRVFPDGN-GAVNEKGLDFYDRLIEELRNHGIEPIVTLYHWDVPQALMDAYGAWESRRIIDDFDRYAVTLFQRFGDRVKYWVTLNEQNIFISF------------------------

General information: TITO was launched using: RESULT: Template: 4ZEN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 641 -66406 -103.60 -428.42 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : -103.60 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.559

(partial model without unconserved sides chains): PDB file : Tito_4ZEN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZEN-query.scw PDB file : Tito_Scwrl_4ZEN.pdb: