Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNLKSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE 1KKL Chain:H ((15-100)) --QKTFKVTADSGIHARPATVLVQTASKYDADVNLEYNGKTVNLKSIMGVMSLGIAKGAEITISASGADENDALNALEETMKSERLGE

General information: TITO was launched using: RESULT: Template: 1KKL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain H - contact count / total energy / energy per contact / energy per residue : 398 -43665 -109.71 -507.73 target 2D structure prediction score : 0.77 Monomeric hydrophicity matching model chain H : 0.94 3D Compatibility (PKB) : -109.71 2D Compatibility (Sec. Struct. Predict.) : 0.77 1D Compatibility (Hydrophobicity) : 0.94 QMean score : 0.556

(partial model without unconserved sides chains): PDB file : Tito_1KKL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KKL-query.scw PDB file : Tito_Scwrl_1KKL.pdb: