Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVSYLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAWGSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSAGDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRMQKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLIPYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTILLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE 5DJQ Chain:M ((53-68)) ---------------------------------------------------------------------------------PLPRWWFLLFIGTLVF---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 5DJQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain M - contact count / total energy / energy per contact / energy per residue : 7 -1008 -144.00 -63.00 target 2D structure prediction score : 0.81 Monomeric hydrophicity matching model chain M : 0.38 3D Compatibility (PKB) : -144.00 2D Compatibility (Sec. Struct. Predict.) : 0.81 1D Compatibility (Hydrophobicity) : 0.38 QMean score : 0.482

(partial model without unconserved sides chains): PDB file : Tito_5DJQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DJQ-query.scw PDB file : Tito_Scwrl_5DJQ.pdb: