Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MCTSITYVTSD--HYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND--LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYK-EIQEGKDNVSPFEFIPWILGQCSTVGEAKKLLKN---INLANINYSDELPLSPLHWLLADKE-KSIVIESMKDGLHIYDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDLSSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSCCNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN 2QUY Chain:A ((1-329)) -CSSLSIRTTDDKSLFARTMDFTMEPDSKVIIVPRNYGIRLLEKENVVINNSYAFVGMGSTDITSPVLYDGVNEKGLMGAMLYYATFATYADEPKKGTRGINPVYVISQVLGNCVTVDDVIEKLTSYTLLNEANIILGFA---PPLHYTFTDASGESIVIEPDKTGITIHRKTIGVMTASPGYEWHQTNLRAYIGVTPNPPQDIMMGDLDLTPFGQGAGGLGLPGDFTPSARFLRVAYWKKYTEKAKNETEGVTNLFHILSSVNIPKGVVLTNEGKTDYTIYTSAMCAQSKNYYFKLYDNSRISAVSLMAENLNSQDLITFEWDRKQDIKQLN

General information: TITO was launched using: RESULT: Template: 2QUY.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1853 30991 16.72 96.85 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain A : 0.80 3D Compatibility (PKB) : 16.72 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.492

(partial model without unconserved sides chains): PDB file : Tito_2QUY.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QUY-query.scw PDB file : Tito_Scwrl_2QUY.pdb: