Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNEKKAVMITKKAGEDMLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVFHPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEETGYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEA-QQAMKDQLICDAKTIYAIQYWELLTKGK 1V8M Chain:A ((11-169)) ------------------------------RTYLYRGRILNLALE---------GRYEIVEHKPAVAVIALRE-GRMLFVRQMRPAVGLAPLEIPAGLIEPGE--DPLEAARRELAEETGL-SGDLTYLFSYFVSPGFTDEKTHVFLAENLKEVEA--HPDEDEAIEVVWMRPEEALERHQRGEVEFSATGLVGVLYYHAFLRG-

General information: TITO was launched using: RESULT: Template: 1V8M.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 722 -65834 -91.18 -416.67 target 2D structure prediction score : 0.40 Monomeric hydrophicity matching model chain A : 0.78 3D Compatibility (PKB) : -91.18 2D Compatibility (Sec. Struct. Predict.) : 0.40 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.334

(partial model without unconserved sides chains): PDB file : Tito_1V8M.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V8M-query.scw PDB file : Tito_Scwrl_1V8M.pdb: