Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MGHSVALNFADGKTFFISVNKDELLLDAAVRQGINLPLDCREGVCGTCQGQCETGIYEQEYVDEDALSERDLAERKMLACQTRVKSDAAFYFDHDSAICNAGDTLKIETQVTAVELVSETTAILHLDASNHAEQLQFLPGQYARLQIPDT--EDWRSYSFANRPNATNQLQFLIRLLPDGVMSNYLRDRCQVGQSLLIEAPLGSFYLRE---VERPLVFVAGGTGLSAFLGMLDNLVEQPNSPTVQFYYGVNSETDLCEQQRLHAYAEQLPNFSYHPIVTKATETWQGKAG-YIHEHLNK-----D---QLAEQAFDMYLCGPPPMIEAVKNWLDEQALQ------NYRIYSEKFLQSNTSR 5UFA Chain:A ((16-267)) --------------------------------------------------------------------------------------------------------KFTEEKILWVKHHTPKLITFAISRP---ESYRFKAGQFSRLGFYEGKGFIWRAYSVVSAEYA-DTLEYFAVLIQDGPMSALFAK-MQQGDTILLDKNATGFLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDRLAALSEH--SFRFVPVTTRAANP-SGLSGKRIPELLKNNSIEQALHTKLTPESTRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF------

General information: TITO was launched using: RESULT: Template: 5UFA.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1183 -8406 -7.11 -37.53 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : -7.11 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.410

(partial model without unconserved sides chains): PDB file : Tito_5UFA.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UFA-query.scw PDB file : Tito_Scwrl_5UFA.pdb: