Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MLTKTVKSFGLAMGLLACSLPLYAKNNVVVVATGGTIAGAGASSANSATYTAAKVPVDALINAVPQIQDLANVSGIQALQVASESITDKELLQIARQVNELVKKPTVNGVVITHGTDTLEETAFFLNLVVHTDKPIVLVGSMRPSTALSADGPLNLYSAVALAASDDAKNKGVMVLMNDSIFAARDVTKGINIHTNAFVSQ-WGALGTLVEGKPYWFRQSVKRHTNASEFNIENIKGDALPTVQIVYGSDSMLPDAYEAYAKAGAKAIIHAGTGNGSVAKYIVPTLQNLHDQNGIQIIRSSRVPQGFVLRDAEQ-PDSKYGWVAAHDLNPQKARLLAALALTKTNDAKEIQRMFWQY 1HO3 Chain:B ((1-325)) ------------------------LPNITILATGGTIAGGGDSATK-SNFTVGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDC--DKTDGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAADKASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARKHTSDTPFDVS--KLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVFDTLATAA-KTGTAVVRSSRVPTGATTQD-AEVDDAKYGFVASGTLNPQKARVLLQLALTQTKDPQQIQQIFNQ-

General information: TITO was launched using: RESULT: Template: 1HO3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1959 -93374 -47.66 -289.08 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -47.66 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.530

(partial model without unconserved sides chains): PDB file : Tito_1HO3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HO3-query.scw PDB file : Tito_Scwrl_1HO3.pdb: