Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKPLLLVFHPDLS--QSKVNRALVATLKDQ-DKIDIVDIQKLY-NGSL----------------------------DIMNDGAIEAQRLISASHIILQFPIYWYSVPPIFKAWMDAVLTRMFYLFYE--QEGQHIEGKPLYLHITVGNLSSAYQKLGQNGFTLDELMAP-LKATARRCGLIWHPPHVIFNANQLNENDITEQTSLSLKQFEQWGIFNNNSFILVLSQKKRTSEKSEVRTML 1D4A Chain:C ((2-195)) GRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIFQFPLQWFGVPAILKGWFERVFIGEFAYTYAAMYDKGPFRSKKAVLSITTGGSGSMYSLQGIH-GDMNVILWPIQSGILHFCGFQVLEPQLTYSIGHT-----------------------------------------------

General information: TITO was launched using: RESULT: Template: 1D4A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 635 -47435 -74.70 -298.33 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain C : 0.67 3D Compatibility (PKB) : -74.70 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.510

(partial model without unconserved sides chains): PDB file : Tito_1D4A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1D4A-query.scw PDB file : Tito_Scwrl_1D4A.pdb: