Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVVVDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKVLGCEGVIVTNAAGGIG--FGPGTLMAISDHINMTG---QNPLMGENLDDFGPRFPDMSRAYTPEYRATAHEVAKKLNI--KLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVAAHSGLKVLGISCITNFAAGFQ---EELNHEEVVEVTERVKGDFKGLLKAILAEL 2P4S Chain:C ((90-367)) -YTYDTLQEIATYLLERTELRPKVGIICGSGLGTLAEQLTDVDSFDYETIPHFPVSTVAGHVGRLVFGYLAGVPVMCMQGRFHHYEGYPLAKCAMPVRVMHLIGCTHLIATNAAGGANPKYRVGDIMLIKDHINLMGFAGNNPLQGPNDERFGPRFFGMANTYDPKLNQQAKVIARQIGIENELREGVYTCLGGPNFETVAEVKMLSMLGVDAIGMSTVHEIITARHCGMTCFAFSLITNMCTMSYEEEEEHCHDSIVGVGKNREKTLGEFVSRIVKHI

General information: TITO was launched using: RESULT: Template: 2P4S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 1489 -109782 -73.73 -409.63 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain C : 0.82 3D Compatibility (PKB) : -73.73 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.82 QMean score : 0.629

(partial model without unconserved sides chains): PDB file : Tito_2P4S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2P4S-query.scw PDB file : Tito_Scwrl_2P4S.pdb: