Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQQVIIVGGGMVGLSLSLMLAKANIAVKLLEAVKYPNYDDQNVAPYHSSFDARN--TALSRRSVQIYQKLGLWDALQQHATPILQVHITEQGSFG-KARLIAEQEKVESFGQVIENAWLGRVLLTQVRQQPLIELIDGVQVTALTQDAEQVHIEAQRGDEVLKLESKLLIAADGRDSFCRQAIGVGVD----VHDYDQVAIVTTVQTSKP---H-EHVGFERFSA-LGPLALLPLPGEYRRSVVWPVKKGTE-----GEWLGE-ENDQHFLDALQKTYGDRAGK---FE-KTGKRFSYPLSQVLAHKQA-VGRIILMGNAAHTIHPVAGQGFNLCLRDADVLLRYLVNQLSASDDIGNPDNLLAYEEARLSDQQRVIKFCDTVVRGFSNQNPLLKLIRNTGLIAFDVIPGVKPLVANYAMGLKA 5FN0 Chain:D ((7-371)) -ARQVTIIGAGLAGTLVARLLARNGWQVNLFERRPDPRI--------ETGARGRSINLALAERGAHALRLAGLEREVLAEAVMMRGRMVHVPGTPPNLQPYG---RDDSEVIWSINRDRLNRILLDGAEAA-GASIHFNLGLDSVDFARQRLTLSNVSG-ERLEKRFHLLIGADGCNSAVRQAMASVVDLGEHLETQPHGYKELQITPEASAQFNLEPNALHIWPHGDYMCIALPNLD-RSFTVTLFLHHQSPAAQPASPSFAQLVDGHAARRFFQRQFPDLSPMLDSLEQDFEHHPTGKLATLRLTTWHVGGQAVLLGDAAHPMVPFHGQGMNCALEDAVALAEHLQSAAD------NASALAAFTAQRQPDALAIQAMALENYV---------------------------------------

General information: TITO was launched using: RESULT: Template: 5FN0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1936 -10508 -5.43 -30.73 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain D : 0.66 3D Compatibility (PKB) : -5.43 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.485

(partial model without unconserved sides chains): PDB file : Tito_5FN0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5FN0-query.scw PDB file : Tito_Scwrl_5FN0.pdb: