Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKVQVKLLDPRLGKEWPLPSYATAGSAGLDLRACLDEAIEIEPGQTVLVKTGMAIYIHDVNFAGLILPRSGLGHKHGIVLGNLVGLIDSDYQGELMVSVWNRGQTT-FRLEPGERLAQYVLVPVVQAEFEQVEEFEETLRGAGGFGHTGKQ 1SJN Chain:B ((9-133)) ----------RLDPGLPLPSRAHDGDAGVDLYSA--EDVELAPGRRALVRTGVAVAV-PFGMVGLVHPRSGLATRVGLSIVNSPGTIDAGYRGEIKVALINLDPAAPIVVHRGDRIAQLLVQRVELVELVEVSSFDEA-------------

General information: TITO was launched using: RESULT: Template: 1SJN.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 524 -57168 -109.10 -461.03 target 2D structure prediction score : 0.48 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -109.10 2D Compatibility (Sec. Struct. Predict.) : 0.48 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_1SJN.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1SJN-query.scw PDB file : Tito_Scwrl_1SJN.pdb: