Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLKRATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDKRIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQEDTFDSLEEKIHRVEHRIYPEVISEIIENGGYLK 3DA8 Chain:B ((13-201)) ARLVVLASGTGSLLRSLLDAAVG-DYPARVVAVGVDR-ECRAAEIAAEASVPVFTVRLADHPSRDAWDVAITAATAAHEPDLVVSAGFMRILGPQFLSRFYGRTLNTHPALLPAFPGTHGVADALAYGVKVTGATVHLVDA--DTGPILAQQPVPVLDGDDEETLHERIKVTERRLLVAAVAALATHGVTV-

General information: TITO was launched using: RESULT: Template: 3DA8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 971 -150279 -154.77 -803.63 target 2D structure prediction score : 0.72 Monomeric hydrophicity matching model chain B : 0.78 3D Compatibility (PKB) : -154.77 2D Compatibility (Sec. Struct. Predict.) : 0.72 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.635

(partial model without unconserved sides chains): PDB file : Tito_3DA8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3DA8-query.scw PDB file : Tito_Scwrl_3DA8.pdb: