Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKNEKKAVMITKKAGEDMLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVFHPGGVGIIAFDEQD-RLLLVKQFRKPLEKVILEIPAGKID-PGEGQNPEMTAARELEEETGYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQQAMKDQLICDAKTIYAIQYWELLTKGK 5I8U Chain:C ((5-182)) ------------------------DFETISSETLHTGAIFALRRDQVRMPGGGIVTREVVEHFGAVAIVAMD-DNGNIPMVYQYRHTYGRRLWELPAGLLDVAGE--PPHLTAARELREEVGLQASTWQVLVDLDTAPGFSDESVRVYLATGLREVGRPEAHHEQADMTMGWYPIAEAARRVLRGEIVNSIAIAGVLAVHAVTTG-

General information: TITO was launched using: RESULT: Template: 5I8U.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 788 -19131 -24.28 -108.70 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain C : 0.78 3D Compatibility (PKB) : -24.28 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.78 QMean score : 0.417

(partial model without unconserved sides chains): PDB file : Tito_5I8U.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I8U-query.scw PDB file : Tito_Scwrl_5I8U.pdb: