Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKTETTIQGLAASLNPARAARKIIGFVPTMGNLHEGHLTLVREAKKLCDVVVVSIFVNPTQFGPGEDFDNYPRTLEQDSRLLADVGCDIIFAPSVEQMYGTQPRLTNISV-SQITDDLCGSSRPGHFDGVALVVTKLFNIVQPNYAFFGQKDYQQLAVIRQFVQDLNIPLEVIGVPIVRAEDGLALSSRNGYLTPEQRQVAPVIYQGLKQAEEQLHQGKDLQQVLADLKTLLTD-NGFVVDYVEARQPNLLAASQ-FDRDIVLFVAAKLGGTRLIDNLQVAFTPQ 1V8F Chain:B ((1-275)) MRTVSTVAELRAALP-----REGVGFVPTMGYLHRGHLALVERARRENPFVVVSVFVNPLQFGPGEDYHRYPRDLERDRALLQEAGVDLLFAPGVEEMYPE-GFATRVQVEGPLTALWEGAVRPGHFQGVATVVARLFLLVQPQRAYFGEKDYQQLLVVRRMVRDLGFPVEVVGVPTVREEDGLALSSRNVYLSPETRKKAPVLYRALLAMREVAGQGGSVAEALRAGEEALRAVPEFRKDYLAIVHPETLLPLSDWVAGARGIVAGRFPEARLIDNLEVY----

General information: TITO was launched using: RESULT: Template: 1V8F.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1437 -91650 -63.78 -336.95 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain B : 0.83 3D Compatibility (PKB) : -63.78 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.83 QMean score : 0.606

(partial model without unconserved sides chains): PDB file : Tito_1V8F.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1V8F-query.scw PDB file : Tito_Scwrl_1V8F.pdb: