Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSGSTITPWVVGNWKMNPMRANANQLIEEFKQLLQQNQIADENCHVGVAP---VSIALTTVQAQLQDAARTVYTVAQDVSRVAGTGAYTGEVSAELLKDSQINFVLVGHSERRDIFGDNVEILKAKLQNALNAGMTVIYCVGESLEQREQGQAEQVVLQQICDIAPVVTAEQWQNQVVIAYEPIWAIGTGKTASPQDAQAMHAKIREGL-CQLTPA-GSNIAILYGGSVKAENAVELAACPDINGALVGGASLNAASFYQIVQAFAQSK 1TPU Chain:B ((6-245)) --------FVGGNWKMNGDKKSLGELI----HTLNGAKLSADTEVVCGAPSIYLDFARQKLDAKIGVAAQNCYKVPK--------GAFTGEISPAMIKDIGAAWVILGNSERRHVFGESDELIGQKVAHALAEGLGVIACIGEKLDEREAGITEKVVFEQTKAIADNV--KDW-SKVVLAYEPVWAIGTGKTATPQQAQEVHEKLRGWLKTHVSDAVAQSTRIIYGGSVTGGNCKELASQHDVDGFLVGGASLK-PEFVDIINA-----

General information: TITO was launched using: RESULT: Template: 1TPU.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1265 -20936 -16.55 -89.09 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain B : 0.81 3D Compatibility (PKB) : -16.55 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.586

(partial model without unconserved sides chains): PDB file : Tito_1TPU.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1TPU-query.scw PDB file : Tito_Scwrl_1TPU.pdb: