Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQQQVIIVGGGMVGLSLSLMLAKANIAVKLLEAVKYPNYDDQNVAPYHSSFDARNTALSRRSVQIYQKLGLWDALQQHATP-ILQVHITEQGSFGKARLIAEQEKVESFGQVIENAWLGRVLLTQVRQQPLIELIDGVQVTALTQDAEQ--VHIEAQRGDEVLKLESKLLIAADGRDSFCRQAIGV-GVDVHDYDQVAIVTTVQTSKPHEHVGFERF-SALGPLALLPLPGEYRRSVVWPVKKGTEGEW-LGEENDQHFLDALQKTYGDRAG-KF---EKTGKRFSYPLSQVLAHKQAVGRIILMGNAAHTIHPVAGQGFNLCLRDADVLLRYLVNQLSASDDIGNPDNLLAYEEARLSDQQRVIKFCDTVVRGFSNQNPLLKLIRNTGLIAFDVIPGVKPLVANYAMGLKA 2X3N Chain:A ((5-370)) NHIDVLINGCGIGGAMLAYLLGRQGHRVVVVEQARRE---------------NGADLLKPAGIRVVEAAGLLAEVTRRGGRVRHELEVYHDGELL-RYFNYSSVDARGYFILMPCESLRRLVLEKIDGEATVEMLFETRIEAVQRDERHAIDQVRLNDG---RVLRPRVVVGADGIASYVRRRLLDIDVERRPYPSPMLVGTFALAPCVAERNRLYVDSQGGLAYFYPIGF-DRARLVVSFPREE-ARELMADTRGESLRRRLQRFVGDESAEAIAAVTGTSRFKGIPIGYLNLDRYWADNVAMLGDAIHNVHPITGQGMNLAIEDASALADALDLALRDAC--ALEDALAGYQAERFPVNQAIVSYGHALATSLEDRQR-FAGVFD-------------------------

General information: TITO was launched using: RESULT: Template: 2X3N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2060 -39801 -19.32 -112.75 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.70 3D Compatibility (PKB) : -19.32 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.498

(partial model without unconserved sides chains): PDB file : Tito_2X3N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2X3N-query.scw PDB file : Tito_Scwrl_2X3N.pdb: