Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSIPQSLLEQSIQINIQQALQEDIGDGDITAMLTPEDEQATATIISREDMVLAGQPWVNALISAYDNTVQVTWLKQEGDRVAANEAFLKLAGSARSLLTVERPALNFIQTLSAVATKTAEYVQHLEGLNTKLLDTRKTLPGLRIAQKYAVTVGGGQNHRLGLFDAFLIKENHIMAAGGIAQAIAKAHQI-APGKPVEVEVETWDELNQALEAGADIVMLDNFSQQQMIDAVKHVAGRCKLEASGNITIENLREVATTGVDYISMGVLTKDVKAVDLSMRFNA 3TQV Chain:B ((12-286)) ----NKVPNDIVTRLVRESLAEDIATGDITAQLA-EDIDTTAFCITREEMILCGQDFANEVINQLDKNIQITWLYSDAQKVPANARIFELKGNVRSILTAERTILNFIQMLSGTATVTNKLVKLISQYKTKLLDTRKTIPGFRLAQKYAVRCGGGFNHRIGLFDAYLIKENHIRSAGGIAKAVTKAK-KLDSNKVVEVEVTNLDELNQAIAAKADIVMLDNFSGEDIDIAVSIARGKVALEVSGNIDRNSIVAIAKTGVDFISVGAITKHIKAIDLSLQVQ-

General information: TITO was launched using: RESULT: Template: 3TQV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1417 -78441 -55.36 -286.28 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.84 3D Compatibility (PKB) : -55.36 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.84 QMean score : 0.447

(partial model without unconserved sides chains): PDB file : Tito_3TQV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TQV-query.scw PDB file : Tito_Scwrl_3TQV.pdb: